Ramulus Cinnamomi essential oil exerts an anti-inflammatory effect on RAW264.7 cells through N-acylethanolamine acid amidase inhibition
Laboratory studyHumansOther animals
- Design
- Laboratory study
- Subjects
- HEK293 human cells overexpressing NAAA and RAW264.7 mouse macrophages stimulated with LPS
- Dose used in the study
- Ramulus Cinnamomi essential oil; palmitoylethanolamide itself was not added
- Duration
- Not stated in the abstract
- What was measured
- NAAA enzyme activity, cellular PEA and OEA levels, nitric oxide and TNF-alpha in macrophages, oil composition by GC-MS and docking of cinnamaldehyde to NAAA.
What the authors reported
This laboratory study found:
- The essential oil inhibited NAAA with an IC50 of 5.64 micrograms/mL and raised PEA and OEA in NAAA-overexpressing cells.
- It reduced nitric oxide and TNF-alpha in LPS-stimulated macrophages.
- (E)-cinnamaldehyde made up 64.88% of the oil and inhibited NAAA with an IC50 of 3.21 micrograms/mL.
- O-methoxycinnamaldehyde had an IC50 of 9.62 micrograms/mL.
Limits of this study
A cell study; it cannot show effect in people or animals. PEA was raised indirectly by blocking its breakdown, not given. The authors declare no competing interests.
Source
PubMed 37311500 · doi:10.1016/j.jep.2023.116747
Entry checked against the abstract on PubMed on 2026-09-22. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.