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Assay of NAAA Activity

Kazuhito Tsuboi, Natsuo Ueda. Methods Mol Biol. 2023:2576:261-274.

Laboratory studyOther animals

Design
Laboratory study
Subjects
Not applicable; a laboratory methods chapter
Dose used in the study
Not applicable; radiolabelled palmitoylethanolamide is used as the enzyme substrate
Duration
Not applicable
What was measured
A protocol for assaying NAAA enzyme activity using [14C]palmitoylethanolamide and thin-layer chromatography, including enzyme preparation and substrate synthesis.

What the authors reported

NAAA prefers palmitoylethanolamide as a substrate at acidic pH, and NAAA inhibitors show anti-inflammatory and analgesic actions in animal models, the chapter reports. It presents an assay method rather than experimental results.

Limits of this study

A methods chapter, not a study of PEA's effects; it cannot show effect in people or animals. Funding and conflicts not stated in the abstract.

Source

PubMed 36152194 · doi:10.1007/978-1-0716-2728-0_22

Entry checked against the abstract on PubMed on 2026-09-22. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.