The Effects of Palmitoylethanolamide or Ibuprofen on the Abundance Profile and Synthesis Rate of Proteins in C2C12 Skeletal Myotubes
Laboratory studyOther animals
- Design
- Laboratory study
- Subjects
- Differentiated C2C12 mouse skeletal myotubes (cell culture)
- Dose used in the study
- PEA 10 micromolar or ibuprofen 100 micromolar, treated for 36 hours
- Duration
- 36 hours
- What was measured
- Protein synthesis rates (1541 proteins) and relative protein abundance (3085 proteins) using D2O labelling, comparing PEA (10 µM) and ibuprofen (100 µM) with vehicle control over 36 hours.
What the authors reported
PEA increased synthesis of 101 proteins and reduced synthesis of 2, and its abundance differences were largely restricted to ribosomal proteins; ibuprofen increased synthesis of 165 proteins and reduced 7, with broader changes across muscle contraction, extracellular matrix, carbohydrate and lipid metabolism proteins, including reduced abundance of 21 carbohydrate-metabolism proteins and increased abundance of 17 lipid-metabolism proteins. Both increased total ribosomal protein synthesis by about 80% and 40S ribosomal subunit abundance by about 18% relative to control.
Limits of this study
A cell culture study in mouse myotubes; it cannot show effect in muscle in a living animal or person. The authors declare no conflicts of interest.
Source
PubMed 42038098 · doi:10.1096/fba.2025-00286
Entry checked against the abstract on PubMed on 2026-10-19. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.