Palmitoylethanolamide (PEA) regulates cell cycle progression and promotes an anti-inflammatory transcriptomic signature in C2C12 skeletal muscle cells
Laboratory studyOther animals
- Design
- Laboratory study
- Subjects
- Differentiated C2C12 mouse myotubes and myoblasts (cell culture)
- Dose used in the study
- PEA 10 micromolar
- Duration
- 24 hours (myoblast cell-cycle analysis); acute treatment for transcriptomics
- What was measured
- Myotube number, fusion index and area; myoblast cell cycle phases; RNA sequencing of differentially expressed genes; NAAA and FAAH expression.
What the authors reported
The study found:
- PEA reduced myotube number (90.3±10.6 vs 112.6±10.1 control) and increased nuclear fusion index (37.8±5.7% vs 30.7±3.2%), with myotube area unchanged.
- In myoblasts, PEA increased G0/G1 cells (48.2±1.2% vs 42.3±1.9%) and reduced S-phase cells (21.7±1.2% vs 25.5±1.2%).
- RNA sequencing found 1952 differentially expressed genes, with PEA lowering NF-kappa-B target cytokines and raising interferon-related and chemokine genes.
- NAAA, not FAAH, was the main enzyme induced.
Limits of this study
A mouse muscle cell culture study; it cannot show effect in muscle in a living animal or person. Conflicts of interest not stated in the abstract.
Source
PubMed 41693292 · doi:10.14814/phy2.70780
Entry checked against the abstract on PubMed on 2026-09-22. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.