Design, Characterization, and In Vitro Assays on Muscle Cells of Endocannabinoid-like Molecule Loaded Lipid Nanoparticles for a Therapeutic Anti-Inflammatory Approach to Sarcopenia
Laboratory studyOther animals
- Design
- Laboratory study
- Subjects
- Mouse C2C12 myoblast cells
- Dose used in the study
- Palmitoylethanolamide loaded in solid lipid nanoparticles, added to cell culture; concentration not stated in the abstract
- Duration
- Up to 14 hours of incubation
- What was measured
- Nanoparticle size, encapsulation efficiency, physical state of PEA, dissolution, cell uptake and toxicity in myoblasts.
What the authors reported
The study found:
- Nanoparticles were about 250 nm with 90% encapsulation efficiency.
- PEA was in an amorphous state inside them, which improved dissolution.
- Uptake by myoblasts was 85 to 94% after 14 hours, with practically no toxicity.
- Particles were seen in the cytoplasm, not the nucleus.
Limits of this study
A formulation study on mouse muscle cells in culture; it cannot show effect in people or animals treated in practice, and no anti-inflammatory outcome was tested. The authors declare no conflict of interest.
Source
PubMed 35336022 · doi:10.3390/pharmaceutics14030648
Entry checked against the abstract on PubMed on 2026-09-22. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.