Palmitoylethanolamide counteracts substance P-induced mast cell activation in vitro by stimulating diacylglycerol lipase activity
Laboratory studyOther animals
- Design
- Laboratory study
- Subjects
- Rat basophilic leukemia (RBL-2H3) cells, a mast cell model, stimulated with substance P
- Dose used in the study
- PEA applied to cell cultures, with or without a CB2 antagonist (AM630) or a diacylglycerol lipase inhibitor (OMDM188); concentrations not stated in the abstract
- Duration
- Not stated in the abstract
- What was measured
- Histamine release (ELISA), degranulation (beta-hexosaminidase release, toluidine blue staining), 2-AG levels (LC-MS), and mRNA of cannabinoid/PEA-related receptors and enzymes
What the authors reported
Substance P increased mast cell degranulation and histamine release. PEA counteracted both effects, an action blocked by the CB2 antagonist AM630. PEA also raised 2-AG levels, reversed by the DAGL inhibitor OMDM188, and directly stimulated DAGL-alpha and DAGL-beta enzyme activity in cell-free systems.
Limits of this study
A cell-based laboratory study; it cannot show effect in people or animals. Three authors are employees of Epitech Group SpA and one is a co-inventor on PEA patents.
Source
PubMed 31878942 · doi:10.1186/s12974-019-1671-5
Entry checked against the abstract on PubMed on 2026-09-22. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.