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Time-controlled online SPE: strategic approach for N-acylethanolamines quantification in complex matrices

Gatti N, Greco V, Cuzzocrea S, Crupi R, Gugliandolo E, Giuffrida A. J Chromatogr A. 2025;1763:466449.

Laboratory studyHorses

Design
Laboratory study
Subjects
Equine plasma samples; number of horses not stated in the abstract
Dose used in the study
No PEA given. An analytical method for measuring the horse's own PEA and oleoylethanolamide in plasma
Duration
Not applicable
What was measured
Detection and quantification limits, recovery and linearity of a liquid chromatography mass spectrometry method; endogenous plasma levels.

What the authors reported

The method measured PEA in horse plasma down to a detection limit of 0.27 ng/mL and quantification limit of 0.83 ng/mL (oleoylethanolamide: 0.04 and 0.11 ng/mL), and reported what the authors describe as the first data on endogenous PEA and oleoylethanolamide levels in horses.

Limits of this study

A methods paper. It gives no treatment data and the endogenous values are not in the abstract. Listed because published horse data on this molecule are scarce; see the horses page. The conflict statement says Valentina Greco reports something provided by the University of Catania (not specified); the other authors declare none.

Source

PubMed 41075686 · doi:10.1016/j.chroma.2025.466449

Entry checked against the abstract on PubMed on 2026-10-19. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.