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Palmitoylethanolamide and Cannabidiol Prevent Inflammation-induced Hyperpermeability of the Human Gut In Vitro and In Vivo: A Randomized, Placebo-controlled, Double-blind Controlled Trial

Couch DG, Cook H, Ortori C, Barrett D, Lund JN, O'Sullivan SE. Inflamm Bowel Dis. 2019;25(6):1006-1018.

Randomised controlled trialHumans

Design
Randomised controlled trial
Subjects
Healthy volunteers taking 600 mg aspirin to raise gut permeability; the abstract does not give the number. Also Caco-2 cell cultures and human colon tissue from bowel resections
Dose used in the study
Oral PEA or cannabidiol or placebo in the human arm; dose not stated in the abstract
Duration
Single-dose lactulose-mannitol permeability test
What was measured
Urinary lactulose and mannitol after aspirin (in vivo); dextran flux across inflamed Caco-2 monolayers; claudin, receptor and aquaporin expression in human colon mucosa

What the authors reported

The trial found:\n- Aspirin raised absorption of lactulose and mannitol, and PEA or cannabidiol reduced that rise (p < 0.001).\n- In cell culture, PEA reduced inflammation-induced dextran flux through a PPAR-alpha dependent route.\n- In human mucosa, PEA prevented the inflammation-induced fall in TRPV1 and rise in PPAR-alpha transcription.

Limits of this study

Abstract only; the number of volunteers, the PEA dose and the crossover details are not given there. The in vivo model is aspirin-induced permeability in healthy people, not inflammatory bowel disease. Mechanistic study; no symptom outcomes.

Source

PubMed 30806665 · doi:10.1093/ibd/izz017

Entry checked against the abstract, supplied by Devlin. Sample size and dose not in the abstract on 2026-09-21. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.