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Astrocytic palmitoylethanolamide pre-exposure exerts neuroprotective effects in astrocyte-neuron co-cultures from a triple transgenic mouse model of Alzheimer's disease

Sarah Beggiato, Tommaso Cassano, Luca Ferraro, Maria C Tomasini. Life Sci. 2020;257:118037.

Laboratory studyOther animals

Design
Laboratory study
Subjects
Cortical astrocyte-neuron co-cultures from 3xTg-AD mice and wild-type mice
Dose used in the study
Palmitoylethanolamide 0.1 micromolar applied to astrocytes for 24 hours before co-culture
Duration
24-hour pre-exposure
What was measured
Neuronal viability, apoptotic nuclei, MAP2-positive cells, neurite aggregations, dendritic branches, cell body area, longest dendrite length and neurites per neuron.

What the authors reported

This lab study found:

  • 3xTg-AD neurons co-cultured with 3xTg-AD astrocytes showed more apoptotic nuclei, less MAP2 and morphological changes than wild-type co-cultures.
  • These signs were significantly reduced when the astrocytes had been pre-exposed to PEA.
  • Pre-exposing astrocytes to amyloid-beta-42 had no effect in the 3xTg-AD co-cultures.

Limits of this study

A cell culture study from a mouse model; it cannot show effect in people. No numbers are given in the abstract. The authors declare no conflict of interest.

Source

PubMed 32622942 · doi:10.1016/j.lfs.2020.118037

Entry checked against the abstract on PubMed on 2026-09-22. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.